+91-0135-2522110, 2522109
MEDICAL COLLEGE
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Address
Patel Nagar, Dehradun-248001,
Uttarakhand, India
Phone No: +91-0135-2522110, 2522109
Fax No: +91-135-2522117
(Principal Office)
E-Mail: principal.sgrrimhs@sgrru.ac.in
Shri Guru Ram Rai Institute of Medical and Health Sciences Map
Medical College Central Molecular Diagnostics Tests

Central Molecular Research Laboratory (CMRL), Biochemistry Department SGRRIM&HS & SMI Hospital, Patel Nagar, Dehradun- U.K. Test Menu

Sr.No Name of the Molecular Diagnostic Test

Rate per test

Clinical Significance

1

Mycobacterium Tuberculosis PCR (TB PCR)

1500-/

Detect bacilli which are members of the Mycobacterium tuberculosis complex i.e. M. tuberculosis, M. bovis and some strains of BCG of the Indian subcontinent.

2

Human Leukocyte Antigen B-27 PCR

(HLAB-27 PCR)

1500-/

Proper prognosis in Ankylosing Spondylitis, juvenile rheumatoid arthritis (JRA), and Uveitis Patients is clinically significant and thus the role of HLA-B27 is an important parameter to evaluate and follow up the patients.

3

Human Papilloma Virus DNA (High Risk Types) PCR

1500-/

For the screening of O ncogenic HPV , HPV-16 and HPV-18 and 16 high- risk HPV types (26,31,33,35,39,45,51,52,53,56,58,59,66,68,73,82) in liquid based cervical cytology specimens and cervical swabs.

4

HIV Quantitative PCR (HIV RNA QUANT.)

4000-/

To assess patient by measuring the baseline HIV RNA level or to monitor the effects of antiretroviral therapy by measuring changes in plasma HIV RNA levels during the course of treatment .The test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV infected patients.

5

Hepatitis C Virus Quantitative Test (HCV RNA QUANT.)

5000-/

Quantitation of Hepatitis C Virus (HCV) RNA genotypes 1 through 6 in human serum or plasma, detection and quantitation of HCV RNA by Polymerase Chain Reaction offers a measure of active viremia. Using PCR, it is possible to detect HCV viremia prior to immunological sero-conversion and to detect changes in the viral load in antibody-positive chronic HCV infected patients undergoing therapy with interferon.

6

Hepatitis C Virus Genotyping Test (HCV Genotyping Test)

5500-/

Detect HCV genotypes/ subtypes 1a, 1b, 2, 3a, 4, 5a, and 6 in clinical isolates in HCV positive cases. HCV genotype 1 is more difficult to treat than Genotypes 2 and 3 and causes more severe liver disease.

7

Hepatitis C Virus Qualitative Test

(HCV RNA QUAL.)

2500-/

Detection of HCV by PCR is a important marker of current infection, as PCR is able to amplify HCV RNA directly, independent of the patient’s immunological status. Using PCR, it has been possible to detect HCV viremia prior to immunological sero-conversion and to detect fluctuation of viremia in antibody-positive chronic HCV patients undergoing therapy.

8

Hepatitis B Virus Quantitative Test (HBV DNA QUANT.)

5000-/

The most direct and reliable measurement of viral replication is thought to be quantitation of HBV viral DNA in serum or plasma. A rapid and sustained drop in HBV DNA levels in patients receiving treatment with alpha interferon, Lamivudine or Ganciclovir has been shown to be a predictive factor for a favorable treatment outcome. Monitoring of HBV DNA levels can predict the development of resistance to Lamivudine. Therefore, a quantitative test for the measurement of HBV DNA is a valuable tool that can be used in conjunction with other serological markers in the management of HBV infection.

9

Hepatitis B Virus Qualitative Test (HBV DNA QUAL.)

2500-/

Detection of HBV DNA is the most reliable estimator of infectivity and viral replication. The most significant weakness of the standard serological tests from the clinical perspective, however, is that they provide no reliable measure of viral replication or viremia in a patient known to have infected with HBV. HBV DNA Viral load can be further estimated for the proper management.

10

Herpes Simplex Virus 1&2 PCR

2500-/

Viral meningitis, commonly caused by either enteroviruses or HSV is more reliably detected by PCR when compared to culture and in a shorter time (one versus up to five days). Genital ulceration usually due to HSV type 2 infections is now routinely detected by PCR. Most painful and annoying recurrent genital herpes is due to HSV 2, and almost all recurrent cold sores or fever blisters are due to HSV 1. However, genital herpes also can be caused by HSV 1.

11

Cytomegalo Virus-Quantitative PCR

5000-/

In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid.

12

Cytomegalo Virus-Qualitative PCR

2500-/

13

BCR/ABL quantitative Test

4000-/

This reverse-transcription PCR-based assay detects the BCR-ABL1 transcript produced by the t(9;22) chromosomal translocation associated with chronic myelogenous leukemia (CML) and a subset of lymphoblastic leukemias. For the P190 transcript associated with the minor t(9;22) breakpoint in lymphoblastic leukemia, BCR-ABL1 transcript levels are expressed as a percent ratio of BCR-ABL1 to the normalizing ABL1 transcript. For the P210 transcript associated with CML, quantitation is further adjusted to the international scale (IS) to allow comparison with other IS-compliant BCR-ABL1 assays. Optimal therapy in CML is associated with transcript levels below the major molecular response (MMR) milestone indicated by a BCR-ABL1/ABL1 % (IS) below 0.1.

14

BCR/ABL qualitative Test

3000-/

The qualitative BCR-ABL test can determine the specific type (isoform) of the Philadelphia chromosome present which is important for appropriate diagnosis and treatment. Qualitative BCR-ABL test detects the presence of the p190, p210 and p230 isoforms; however, the qualitative test does not measure the levels of the transcripts.

15

Mycobacterium tuberculosis/Non Tuberculosis differentiation test

2000-/

Simultaneous detection and differentiation of MTB and NTM in a single test

16

HINI (Swine Flu) Qualitative Real Time PCR Test*.

4500-/

Useful for the Detection and Differentiation of Influenza A and HINI Influenza A (H1N1, 2009)
Design & Developed by: IT Department Shri Guru Ram Rai Institute of Medical & Health Sciences & Shri Mahant Indiresh Hospital